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Objective@#The random forest algorithm was used to construct a rapid screening diagnostic prediction model for children with autism spectrum disorder, to provide the references for early detection, early diagnosis of ASD children, and to reduce the pressure of ASD clinical diagnosis and assessment.@*Methods@#The random forest algorithm of machine learning was applied to build the auxiliary diagnosis model. Totally 346 ASD children and 90 normal children were evaluated by Social Responsiveness Scale and Vineland Adaptive Behavior Scales. ROC curve, and accuracy was used to evaluate the models.@*Results@#Among the models, the accuracy of 13 feature factors and 7 feature factors were above 0.9, the sensitivity was up to 0.927, the specificity was up to 0.936 and the AUC was up to 0.979. The accuracy, sensitivity, specificity and AUC of the model were 0.943,0.959,0.931 and 0.978 respectively. The fitting and generalization effects of the three models were all satisfactory.@*Conclusion@#A random forest model based on the SRS Scales and Vineland Adaptive Behavior Scales can be used to diagnose ASD accurately and provide scientific basis for the development of rapid screening and diagnosis tools.
ABSTRACT
Objective@#To investigate knowledge, attitude and behavior related to tuberculosis prevention among primary and elementary school students in Heilongjiang province, and to provide evidence for school-based tuberculosis prevention and control.@*Methods@#A cross-sectional survey method was used to conduct a questionnaire survey among 3 033 middle and elementary school students in 10 counties (districts) of Heilongjiang province, including knowledge, attitudes and behaviors related to tuberculosis prevention and treatment.@*Results@#In the survey, 61.5% of the students understand the core information of tuberculosis. The core knowledge awareness rate was higher in primary school students (64.8%) than in middle school students(58.1%)(χ2=85.41，P<0.05). Primary and middle school students indicated that the attitude-taking rate of concerns about the tuberculosis patients was 47.5%. Primary school students had more general attitude than middle school students towards willingness to participate in publicity activities compared with primary school students(χ2=39.26，P<0.05). About 85% of students reported personal hygiene habits. The results of multiple linear regression analysis showed that tuberculosis-related knowledge was mainly associated with factors including urban and rural, parental education, health education courses in school(P<0.05).@*Conclusion@#There is a gap in tuberculosis-related knowledge among primary and middle school students between Heilongjiang province and national level. There’s a need for the improvement of tuberculosis control programs to inform a better understanding and social dissemination of TB information.
ABSTRACT
The sequences of ITS, matK, rbcL and psbA-trnH of 9 Gynostemma species or variety including 38 samples were compared and analyzed by molecular phylogeny method. Hemsleya macrosperma was designated as outgroup. The MP and NJ phylogenetic tree of Gynostemma was built based on ITS sequence, the results of PAUP phylogenetic analysis showed the following results: (1) The eight individuals of G. pentaphyllum var. pentaphyllum were not supported as monophyletic in the strict consensus trees and NJ trees. (2) It is suspected whether G. longipes and G. laxum should be classified as the independent species. (3)The classification of subgenus units of Gynostemma plants is supported.
Subject(s)
Gynostemma , Classification , Genetics , Molecular Sequence Data , Phylogeny , Plant Proteins , Genetics , Sequence Analysis, DNAABSTRACT
<p><b>OBJECTIVE</b>To clone IspE and IspH unigene from Lonicera japonica and its substitutes, and analyze their gene sequence, protein properties and transcriptional level.</p><p><b>METHOD</b>IspE and IspH unigene ware obtained from the transcriptome dataset of L. japonica. Full-length cDNA of IspE and IspH were cloned from buds of L. japonica, L. japonica var. chinensis, L. hypoglauca and L. dasystyla using RT-PCR technology, and named as LJIspE, LHIspE, LJCIspE, and LDIspE, LJIspH, LJCIspH, LHIspH and LDI-spH, respectively. And we also predicted the structure and function of IspE and IspH proteins.</p><p><b>RESULT</b>IspE contained an open reading frame that consisted of 1 221 bp, encoding one polypeptide with 422 amino acids. A complete open reading frame of IspH gene consisted of 1 380 bp and encoded 459 amino acids. Both IspE and IspH ware non-secreted proteins and localized in the chloroplast. Transcripted level of IspE and IspH in bud of L. japonica, L. hypoglauca and L. dasystyla was not significantly difference, but their transcripted level in L. japonica var. chinensis was significantly higher than that in L. japonica.</p><p><b>CONCLUSION</b>The clone of IspE and IspH will help for further research on the synthesis of terpenes, aroma and color.</p>
Subject(s)
Cloning, Molecular , Computational Biology , Flowers , Chemistry , Genetics , Metabolism , Lonicera , Chemistry , Classification , Genetics , Metabolism , Molecular Sequence Data , Phylogeny , Plant Proteins , Chemistry , Genetics , Metabolism , Protein TransportABSTRACT
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
Subject(s)
Antibodies , Allergy and Immunology , Metabolism , Antibody Specificity , Blotting, Western , Enzyme-Linked Immunosorbent Assay , Escherichia coli , Genetics , Metabolism , Flowers , Chemistry , Gene Expression Regulation, Plant , Genetic Vectors , Lonicera , Chemistry , Phenylalanine Ammonia-Lyase , Chemistry , Allergy and Immunology , Plants, Medicinal , Chemistry , Plasmids , Protein Structure, Secondary , Protein Structure, TertiaryABSTRACT
<p><b>OBJECTIVE</b>To study the developmental phase on the growth and active compounds in Scutellaria baicalensis.</p><p><b>METHOD</b>Seeds of wild plants were collected from Laiwu and sowed in Fangshan (Beijing) and Laiwu (Shandong). Samples of aerial and underground parts were collected in five growth periods of sprouts, seedlings, flowering, seed drop and withered periods respectively. The length of taproot, fresh weight of root, diameter of taproot and the length of stem were determined. The content of active compounds and total flavonoids were determined by HPLC and ultraviolet spectrophotometry respectively. The transcripted level of PAL1, PAL2, PAL3, C4H, 4CL, CHS, GUS and UBGAT were analyzed with RT-PCR.</p><p><b>RESULT</b>The results showed that the aerial part of S. baicalensis grew quickly before flowering stage, and the underground part grew mostly between the periods of flowering and withered. In the whole growing developmental periods, the content of total flavonoids was not changed significantly, the content of baicalin was increased gradually and the content of baicalein was decreased gradually. Expression level of PAL and 4CL was the highest in withered period, CHS was increased between flowering and seed drop and decreased in withered period.</p><p><b>CONCLUSION</b>Seedlings and withered periods may be the key phase affecting the growth and active compounds in S. baicalensis.</p>
Subject(s)
Acyltransferases , Genetics , Metabolism , Chromatography, High Pressure Liquid , Coenzyme A Ligases , Genetics , Metabolism , Flavonoids , Metabolism , Flowers , Genetics , Metabolism , Gene Expression Regulation, Developmental , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Plant , Glucuronidase , Genetics , Metabolism , Glucuronosyltransferase , Genetics , Metabolism , Phenylalanine Ammonia-Lyase , Genetics , Metabolism , Plant Proteins , Genetics , Metabolism , Plant Roots , Genetics , Metabolism , Plant Stems , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Scutellaria baicalensis , Genetics , Metabolism , Seedlings , Genetics , Metabolism , Spectrophotometry, Ultraviolet , Time Factors , Trans-Cinnamate 4-Monooxygenase , Genetics , MetabolismABSTRACT
A FatB unigene was obtained from the transcriptome dataset of Lonicera japonica Thunb. Full-length FatB cDNA was cloned from buds of Lonicera japonica Thunb., Lonicera japonica Thunb. var. chinensis (Wats.) Bak., Lonicera hypoglauca Miq. and Lonicera dasystyla Rehd. using RT-PCR technology, and named as LJFatB, LHFatB, LJCFatB and LDFatB. The results of bioinformatic analysis showed that LJFatB, LJCFatB, LHFatB and LDFatB and Arabidopsis thaliana AtFatB had a closely relationship. Nucleotide sequences and protein secondary structure of LJFatB, LJCFatB, LHFatB and LDFatB are different and their proteins had conserved FatB substrate binding sites and catalytic activity sites. Transcriptive level of LJFatB, LJCFatB, LHFatB and LDFatB in bud was not significantly different. Therefore, LJFatB, LJCFatB, LHFatB and LDFatB could have the same biological function as AtFatB.